Mechanism of protein synthesis inhibition by bottromycin A2: studies with puromycin.

Bottromycin A2 inhibits polylysine synthesis with poly A at low concentrations of E. coli ribosomes. Peptide release by puromycin from ribosomes was significantly inhibited by blasticidin S, chloramphenicol and thiophenicol, but not by bottromycin A2 in the absence of GTPand G factor. Puromycindependent release of peptide from ribosomes was stimulated by the presence of GTP and G factor; it was inhibited by bottromycin A2. The results indicate that bottromycin A2 inhibits translocation of peptidyl-tRNA on the ribosomes, but does not affect peptide bond synthesis.